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目的: 建立快速灵敏的LC-MS/MS法测定人血浆中阿莫西林浓度,并用于两种阿莫西林胶囊的一致性评价。方法: 采用岛津公司LCMS-8060型LC-MS/MS仪,以MRM模式测定阿莫西林(m/z 366.00/114.00)的浓度,d4-阿莫西林作内标(m/z 370.10/114.05),离子源为ESI源。色谱柱选用Waters ACQUITY BEH C18(2.1×50 mm,1.7 μm),梯度洗脱。血浆样本加入内标,经甲醇沉淀蛋白后取上清液进样检测。结果: 所建方法经验证,其线性、准确度、精密度、最低定量限、提取回收率、特异性、基质效应、稳定性等各项指标均符合CFDA的指导原则及最新核查标准要求,并较文献报道中的方法有处理简单、灵敏度高、色谱峰形好的优点。结论: 所建方法快速、灵敏,适用于人血浆中阿莫西林浓度的检测。用于一致性评价的样本实测,两种制剂生物等效。 相似文献
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本文介绍ACS-15电子计价秤示值误差测量结果不确定度的评定方法,并通过实际测量,计算出7.5kg秤量点的标准不确定度、合成标准不确定度及扩展不确定度。 相似文献
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Lucas Zago Naves David‐Alain Gerdolle Oswaldo Scopin de Andrade Marco Markus Maria Gresnigt 《Microscopy research and technique》2020,83(9):1118-1123
In restorative dentistry, the in situ replication of intra‐oral situations, is based on a non‐invasive and non‐destructive scanning electron microscopy (SEM) evaluation method. The technique is suitable for investigation restorative materials and dental hard‐ and soft‐tissues, and its interfaces. Surface characteristics, integrity of interfaces (margins), or fracture analysis (chipping, cracks, etc.) with reliable resolution and under high magnification (from ×50 to ×5,000). Overall the current study aims to share detailed and reproducible information about the replica technique. Specific goals are: (a) to describe detailed each step involved in producing a replica of an intra‐oral situation, (b) to validate an integrated workflow based on a rational sequence from visual examination, to macrophotography and SEM analysis using the replica technique; (c) to present three clinical cases documented using the technique. A compilation of three clinical situations/cases were analyzed here by means the replica technique showing a wide range of possibilities that can be reached and explored with the described technique. This guidance document will contribute to a more accurate use of the replica technique and help researchers and clinicians to understand and identify issues related to restorative procedures under high magnification. 相似文献
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Failures due to intergranular corrosion in components of austenitic stainless steel have always been a tough problem in engineering practice. In this paper, the collinear wave mixing was investigated to evaluate the intergranular corrosion in austenitic stainless steel. An acoustic nonlinearity parameter related to the bispectrum, the propagation distance and the amplitudes of fundamental waves in measured signal is proposed. Nonlinear acoustic measurements were conducted on four tubes with different degree of intergranular corrosion. The experimental results demonstrated that the proposed acoustic nonlinearity parameter is sensitive to intergranular corrosion in samples, and is well correlated with the degree of damage. 相似文献
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This paper reports on the use of eddy current pulsed thermography (ECPT) for detection and characterization of rolling contact fatigue (RCF). Detection mechanisms with eddy currents and heat propagation effects were discussed with RCF modeled as a simple angled defect. Two different angled defects were studied through numerical simulations and experimentally by using uniform magnetic field (UMF) excited by Helmholtz coils. Finally, a rail sample with RCF defects was inspected using UMF excitation. It is shown that ECPT with UMF excitation provides an efficient and robust method to detect angled defects, compared with nonuniform magnetic field (NUMF) excitation. 相似文献